Single-cell gene expression
Transcriptomics Single-cellhttps://pmc.ncbi.nlm.nih.gov/articles/PMC9479272/
Whole transcriptome single-cell gene expression. This assay can be obtained through several technologies and protocols, depending on the experimental design, goals to achieve, the required readout and sensitivity.
Production systems
10xGenomics Chromium
10X Chromium is a platform developed by 10X Genomics for high-throughput single-cell RNA sequencing (scRNA-seq). It enables the profiling of whole transcriptome gene expression in thousands of individual cells simultaneously. It captures single cells in droplets, barcoding each cell's RNA with unique molecular identifiers (UMIs), and sequencing the RNA to provide a detailed transcriptomic profile of each cell. This technology is crucial for studying cellular heterogeneity, identifying rare cell types, and understanding complex biological systems.
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droplet-based single-cell RNAseq
The analysis of single-cell gene expression data generated by droplet-based systems such as the 10X Genomics Chromium system involves several key steps. First, raw sequencing data are processed to generate gene expression matrices, which quantify the expression levels of genes in individual cells. Next, several data transformation steps are carried out to extract the biological variability that distinguishes communities of cells that share a common transcriptomic identity, reflecting their function or responses to stimuli. Finally, several statistical analyses can be carried out to quantify changes in tissue distribution or expression profiles for each of the populations identified, between different experimental conditions.
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